Your mission will consist of preparing, maintaining, and manipulating primary astrocyte cultures in an epileptogenic environment using pharmacological and genetic tools. The impact of these manipulations on astrocyte functions, under environmental conditions mimicking epilepsy in vitro, will be studied using molecular biology techniques such as ELISA, qPCR, Western blotting, and immunocytochemistry.
The volunteer will also be trained in the handling of laboratory mice in order to collect the brain tissues required for the preparation of cell cultures. They will be expected to troubleshoot technical issues during the development of the cultures, write analytical reports in English with a critical mindset, and interpret the data.