Research Assistant - Expression Profiling
Evotec
Goettingen, Germany
Main responsibilities:
- Isolate RNA and DNA from cell cultures, tissue and blood using manual protocols as well as laboratory automation;
- Quantify and quality control isolated nucleic acids using spectrophotometric, fluorometric and electrophoretic methods;
- Perform reverse transcription synthesis followed by gene expression analysis via qPCR;
- Perform raw data quality control and analysis;
- Document experiments, use of reagents and consumables for inventory and reordering purposes and communicate minimally with internal clients;
- Perform other molecular biology techniques such as PCR, genotyping, and restriction digestions occasionally;
- Support the Human Sample Management group with isolation of nucleic acids from human blood and tissue samples using automated liquid handlers occasionally.
Qualifications:
- Successfully completed apprenticeship (BTA/CTA/MTA) or bachelor's studies in the field of Natural Sciences, preferably in molecular biology, biotechnology, biochemistry, or related field with hands-on experience in an academic, pharmaceutical, or biotechnology environment or alternatively, a master’s degree in a relevant discipline with proven experimental expertise;
- Experience and confidence in performing molecular biology methods (RNA and DNA isolation, qPCR, PCR agarose gel electrophoresis etc.);
- First-hand experience with laboratory automation or robotics. Ideally, with experience in handling and maintaining a robotic platform with occassional troubleshooting when needed;
- Ability to work collaboratively with others as a true team player;
- Flexible, resourceful, reliable, and a can-do attitude;
- Ability to pay particular attention to detail and keep accurate records.
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